IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/24: Difference between revisions

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(Autocreate 2014/07/24 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b> ==
==<b>Date: 7/24/14</b> <b>People in lab: Emily Puleo</b>==
<b><span style="font-size:16px;">Title:</span></b> PCR hmp
<b>Start Time:</b> 12:03 pm
<b>Purpose:</b> Add prefix and suffix
<b>Protocol:</b> LTM Ed 2 PCR
<b>Exceptions:</b> 2.5 uL primers, 7.5 uL E coli Template
<b>Products:</b>
{| border= " 1 " cellpadding = " 5 "
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| 7/24 A || hmp with prefix and suffix added || --- || 1


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.


<b>Start Time:</b>


<b>Purpose:</b>


<b>Protocol:</b> <b>Exceptions:</b>


|}
<b>Results:</b>
<b>Notes:</b>
<b>Stop Time:</b> 3:10 pm
<b>Next:</b> TOPO
==<b>Date: 7/24/14</b> <b>People in lab: Emily Puleo, Caleb Treccazi</b>==
<b><span style="font-size:16px;">Title:</span></b> TOPO cloning of hmp and transformations of hmp and norV
<b>Start Time:</b> 3:10 pm
<b>Purpose:</b> To get lots of copies of hmp and do blue white screening and to test ligated norV product
<b>Protocol:</b> LTM Ed 2 Transformations, Invitrogen TOPO cloning kit manual
<b>Exceptions:</b>
<b>Products:</b>  
<b>Products:</b>  
{| border="1" cellpadding="5"
{| border= " 1 " cellpadding = " 5 "  
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity  
|-
|-
| Entry || Entry || Entry || #
| 7/24 CT hmp 1 || 20 uL hmp and 60 uL xgal || 7/24 A || 1
|-
|-
| Entry || Entry || Entry || #
| 7/24 CT hmp 2 || 200 uL hmp and 60 uL xgal || 7/24 A || 1
|}
 
{| border="1" cellpadding="5"
|-
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
| 7/24 CT norV 1 || 20 uL || L1 6/6 || 1
|-
|-
| Sample || || || ||  ||  ||  ||  ||
| 7/24 CT norV 2 || 200 uL || L1 6/6 || 1
|}


<b>Results:</b>


|}
<b>Results:</b> (7/25) No growth except on hmp 1 where there were lots of white colonies and one blue colony
<b>Notes:</b>  
<b>Notes:</b>  
 
<b>Stop Time:</b> 5:45 pm
<b>Stop Time:</b>  
<b>Next:</b> blue white screening of hmp; innoculation of norV from any growth
 
<b>Next:</b>
 




Revision as of 21:55, 13 October 2014

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Date: 7/24/14 People in lab: Emily Puleo

Title: PCR hmp Start Time: 12:03 pm Purpose: Add prefix and suffix Protocol: LTM Ed 2 PCR Exceptions: 2.5 uL primers, 7.5 uL E coli Template Products:

Sample Label Description Source Label Quantity
7/24 A hmp with prefix and suffix added --- 1





Results: Notes: Stop Time: 3:10 pm Next: TOPO

Date: 7/24/14 People in lab: Emily Puleo, Caleb Treccazi

Title: TOPO cloning of hmp and transformations of hmp and norV Start Time: 3:10 pm Purpose: To get lots of copies of hmp and do blue white screening and to test ligated norV product Protocol: LTM Ed 2 Transformations, Invitrogen TOPO cloning kit manual Exceptions: Products:

Sample Label Description Source Label Quantity
7/24 CT hmp 1 20 uL hmp and 60 uL xgal 7/24 A 1
7/24 CT hmp 2 200 uL hmp and 60 uL xgal 7/24 A 1
7/24 CT norV 1 20 uL L1 6/6 1
7/24 CT norV 2 200 uL L1 6/6 1


Results: (7/25) No growth except on hmp 1 where there were lots of white colonies and one blue colony Notes: Stop Time: 5:45 pm Next: blue white screening of hmp; innoculation of norV from any growth



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