IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/07/24: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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<b>Stop Time:</b> 3:10 pm
<b>Stop Time:</b> 3:10 pm
<b>Next:</b> TOPO
<b>Next:</b> TOPO
==<b>Date: 7/24/14</b> <b>People in lab: Emily Puleo, Caleb Treccazi</b>==
<b><span style="font-size:16px;">Title:</span></b> TOPO cloning of hmp and transformations of hmp and norV
<b>Start Time:</b> 3:10 pm
<b>Purpose:</b> To get lots of copies of hmp and do blue white screening and to test ligated norV product
<b>Protocol:</b> LTM Ed 2 Transformations, Invitrogen TOPO cloning kit manual
<b>Exceptions:</b>
<b>Products:</b>
{| border= " 1 " cellpadding = " 5 "
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| 7/24 CT hmp 1 || 20 uL hmp and 60 uL xgal || 7/24 A || 1
|-
| 7/24 CT hmp 2 || 200 uL hmp and 60 uL xgal || 7/24 A || 1
|-
| 7/24 CT norV 1 || 20 uL || L1 6/6 || 1
|-
| 7/24 CT norV 2 || 200 uL || L1 6/6 || 1
|}
<b>Results:</b> (7/25) No growth except on hmp 1 where there were lots of white colonies and one blue colony
<b>Notes:</b>
<b>Stop Time:</b> 5:45 pm
<b>Next:</b> blue white screening of hmp; innoculation of norV from any growth


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Latest revision as of 00:24, 27 September 2017

Cyanobacteria Mediated Filtration of Coal Flue Gas Main project page
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Date: 7/24/14 People in lab: Emily Puleo

Title: PCR hmp Start Time: 12:03 pm Purpose: Add prefix and suffix Protocol: LTM Ed 2 PCR Exceptions: 2.5 uL primers, 7.5 uL E coli Template Products:

Sample Label Description Source Label Quantity
7/24 A hmp with prefix and suffix added --- 1





Results: Notes: Stop Time: 3:10 pm Next: TOPO

Date: 7/24/14 People in lab: Emily Puleo, Caleb Treccazi

Title: TOPO cloning of hmp and transformations of hmp and norV Start Time: 3:10 pm Purpose: To get lots of copies of hmp and do blue white screening and to test ligated norV product Protocol: LTM Ed 2 Transformations, Invitrogen TOPO cloning kit manual Exceptions: Products:

Sample Label Description Source Label Quantity
7/24 CT hmp 1 20 uL hmp and 60 uL xgal 7/24 A 1
7/24 CT hmp 2 200 uL hmp and 60 uL xgal 7/24 A 1
7/24 CT norV 1 20 uL L1 6/6 1
7/24 CT norV 2 200 uL L1 6/6 1


Results: (7/25) No growth except on hmp 1 where there were lots of white colonies and one blue colony Notes: Stop Time: 5:45 pm Next: blue white screening of hmp; innoculation of norV from any growth



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