IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2014/02/05
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 Cyanobacteria Mediated Filtration of Coal Flue Gas
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Date: 02/5/14 People in lab: Kelsey CrossenTitle: PCR of nosZ, adding prefix/suffix Start Time: 12:05 PM Purpose: To amplify the nosZ gene and add the iGEM prefix/suffix Protocol: LTM ed. 2 and Dr. Shannon's lab PCR protocol Exceptions: 1. Annealing temp: 50C, 2. Final primer conc. <0.1uM Products:
Notes: Dr Shannon's Protocol: 7.5 uL template, 3 uL DreamTaq buffer, 3 uL dNTPs, 2.5 uL F primer, 2.5 uL R primer, 10.5 uL milliQ, 1 uL DreamTaq. Hot start (95C) then add Taq. Stop Time: Next: Gel electrophoresis of PCR product Date: 02/5/14 People in lab: Levi PalmerTitle: Gel electrophoresis of 2/5 PCR product Start Time: 4:00 PM Purpose: To check for successful PCR Protocol: LTM ed. 2
Results: No product visible Notes: Only 30sec elongation time, extend time and try again. Stop Time: 8:00 PM | |||||||||||||||||||||||||||||||
