IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/09/27
 Cyanobacteria Mediated Filtration of Coal Flue Gas
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Date: 09/27/13 People in lab: Emily PuleoTitle: PCR hmp and norCB for TOPO TA cloning Start Time: 10:15 AM Purpose: To add prefix/suffix to hmp and norCB Protocol: LTM ed. 2 Exceptions: Annealing Temp: 65C, Elongation time 7min Products:
Notes: See 8/6/13 for hmp calculations, 7/31/13 for norCB. Restarted at 12:35PM Stop Time: 3:15 PM Next: TOPO TA cloning and transformation Date: 09/27/13 People in lab: Emily Puleo, Levi PalmerTitle: TOPO TA cloning of hmp and norCB, gel electrophoresis to check for products of PCR Start Time: 4:45 PM Purpose: To check for prescence of genes and amplify by TOPO-TA cloning Protocol: LTM ed. 2, Invitrogen TOPO-TA cloning manual Products:
Results: hmp visible on gel, norCB had no product visible Next: Check for hmp growth, miniprep if present. Redo PCR for norCB Date: 09/27/13 People in lab: Emily Puleo, Levi PalmerTitle: Gel extraction and ligation of backbone Start Time: 6:50 PM Purpose: To extract the hmp from the gel, to convert the linear plasmid backbone to a circular backbone. Protocol: LTM ed. 2 Products:
Notes: Did not digest backbone first, cannot ligate. Stop Time: 8:00 PM Next: Digestion of backbone | |||||||||||||||||||||||||
