IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/06

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Date: 08/06/13 Blythe Ferriere, Annie Goo

Title: People in lab: Dilute primers plus PCR

Start Time: 2:15 PM

Purpose: Dilute primers for PCR reaction of norV gene, PCR norV gene from E. coli K-12 add iGEM prefix-suffix

Protocol: Dilution: Forward Primer: 0.39mg, To make 1 ug/uL: add 390 uL milliQ, To make 10ng/uL: add 1 uL of 1 ug/uL solution to 100 uL milliQ, Final Solution: ~0.7 uM Reverse Primer: 0.38mg, To make 1ug/uL: add 380 uL milliQ, To make 10ng/uL: add 1 uL of 1 ug/uL solution to 100 uL milliQ, Final Solution: ~0.7 uM

Products:

Sample Label Description Source Label Quantity
8/6/13 norV A 1 Amplified norV gene w/ iGEM prefix & suffix using 7.5 uL of template DNA template DNA 7/31/13 3
8/6/13 norV A 4 Amplified norV gene w/ iGEM prefix & suffix using 5 uL of template DNA Template DNA 7/31/13 3

Results: N/A

Notes: Changed programs about 15 minutes in

Stop Time: 5:00 PM

Next: Ligtate into iGEM backbone

Date: 08/06/13 Emily Puleo, Ali


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