IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/06

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(Autocreate 2013/08/06 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
(Date: MM/DD/YY People in lab: Yourname)
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
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== <b>Date: 08/06/13</b> <b> Blythe Ferriere, Annie Goo</b>  ==
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<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
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<b><span style="font-size:16px;">Title:</span></b> People in lab: Dilute primers plus PCR
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<b>Start Time:</b>
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<b>Start Time:</b> 2:15 PM
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<b>Purpose:</b>  
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<b>Purpose:</b> Dilute primers for PCR reaction of norV gene, PCR norV gene from E. coli K-12 add iGEM prefix-suffix
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<b>Protocol:</b> <b>Exceptions:</b>  
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<b>Protocol:</b> Fill in later pg 10 lab notebook  <b>Exceptions:</b>  
<b>Products:</b>  
<b>Products:</b>  
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! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity
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|-
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| Entry || Entry || Entry || #
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| 8/6/13 norV A 1 || Amplified norV gene w/ iGEM prefix & suffix using 7.5 uL of template DNA || template DNA 7/31/13 || 3
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|-
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| Entry || Entry || Entry || #
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| 8/6/13 norV A 4 || Amplified norV gene w/ iGEM prefix & suffix using 5 uL of template DNA || Template DNA 7/31/13 || 3
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<b>Results:</b>  
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<b>Results:</b> N/A
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<b>Notes:</b>  
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<b>Notes:</b> Changed programs about 15 minutes in
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<b>Stop Time:</b>  
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<b>Stop Time:</b> 5:00 PM
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<b>Next:</b>
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<b>Next:</b> Ligtate into iGEM backbone

Revision as of 19:26, 28 January 2014

Cyanobacteria Mediated Filtration of Coal Flue Gas Main project page
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Date: 08/06/13 Blythe Ferriere, Annie Goo

Title: People in lab: Dilute primers plus PCR

Start Time: 2:15 PM

Purpose: Dilute primers for PCR reaction of norV gene, PCR norV gene from E. coli K-12 add iGEM prefix-suffix

Protocol: Fill in later pg 10 lab notebook Exceptions:

Products:

Sample Label Description Source Label Quantity
8/6/13 norV A 1 Amplified norV gene w/ iGEM prefix & suffix using 7.5 uL of template DNA template DNA 7/31/13 3
8/6/13 norV A 4 Amplified norV gene w/ iGEM prefix & suffix using 5 uL of template DNA Template DNA 7/31/13 3

Results: N/A

Notes: Changed programs about 15 minutes in

Stop Time: 5:00 PM

Next: Ligtate into iGEM backbone



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