IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/03
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Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (1/2)
Title: Gel electrophoresis of 8/2/13 A norCB 1 and 2
Start Time: 2:05 PM
Purpose: Confirm norCB gene with iGEM prefix & suffix. Use the better sample to purify and sequence.
Protocol: LTM ed. 2 pg 45-47
Stop Time: 3:30 PM
Next: The ~1900 bp fragment from 8/2/13 A norCB 2 should be extracted and purified.
Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (2/2)
Title: Gel extraction of ~2000bp fragment from today's gel
Start Time: 3:50 PM
Purpose: To extract the PCR product of the norCB gene + iGEM prefix & suffix
Protocol: IBI Gel extraction Exceptions: 1. Milli-Q used instead of Elution Buffer
Stop Time: 4:45 PM
Next: 8/3/13 GE norCB should be ligated into the iGEM vector.