IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/03

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(Autocreate 2013/08/03 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
Current revision (17:59, 28 January 2014) (view source)
(Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (2/2))
 
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
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== <b>Date: 08/3/13</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b>  (1/2) ==
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<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
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<b><span style="font-size:16px;">Title:</span></b> Gel electrophoresis of 8/2/13 A norCB 1 and 2
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<b>Start Time:</b>
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<b>Start Time:</b> 2:05 PM
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<b>Purpose:</b>  
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<b>Purpose:</b> Confirm norCB gene with iGEM prefix & suffix. Use the better sample to purify and sequence.
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<b>Protocol:</b> <b>Exceptions:</b>
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<b>Protocol:</b> LTM ed. 2 pg 45-47 
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<b>Products:</b>  
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{| border="1" cellpadding="5"
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! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
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| Sample || Ladder || 8/2/13 A norCB  1 || 8/2/13 A norCB 2 || || || || ||
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|}
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<b>Products:</b> N/A
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<b>Results:</b> PICTURE
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<b>Notes:</b>
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<b>Stop Time:</b> 3:30 PM
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<b>Next:</b> The ~1900 bp fragment from 8/2/13 A norCB 2 should be extracted and purified.
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== <b>Date: 08/3/13</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b> (2/2) ==
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<b><span style="font-size:16px;">Title:</span></b> Gel extraction of ~2000bp fragment from today's gel
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<b>Start Time:</b> 3:50 PM
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<b>Purpose:</b> To extract the PCR product of the norCB gene + iGEM prefix & suffix
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<b>Protocol:</b> IBI Gel extraction <b>Exceptions:</b> 1. Milli-Q used instead of Elution Buffer
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<b>Products:</b>
{| border="1" cellpadding="5"
{| border="1" cellpadding="5"
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! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity
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| Entry || Entry || Entry || #
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| 8/3/13 GE norCB || norCB w/ iGEM prefix/suffix (~1900 bp) from well 3 || Well 3 8/3/13 Gel ||1
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| Entry || Entry || Entry || #
 
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<b>Results:</b>  
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<b>Results:</b> N/A
<b>Notes:</b>  
<b>Notes:</b>  
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<b>Stop Time:</b>  
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<b>Stop Time:</b> 4:45 PM
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<b>Next:</b>
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<b>Next:</b> 8/3/13 GE norCB should be ligated into the iGEM vector.
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Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (1/2)

Title: Gel electrophoresis of 8/2/13 A norCB 1 and 2

Start Time: 2:05 PM

Purpose: Confirm norCB gene with iGEM prefix & suffix. Use the better sample to purify and sequence.

Protocol: LTM ed. 2 pg 45-47


Well 1 2 3 4 5 6 7 8
Sample Ladder 8/2/13 A norCB 1 8/2/13 A norCB 2

Products: N/A

Results: PICTURE

Notes:

Stop Time: 3:30 PM

Next: The ~1900 bp fragment from 8/2/13 A norCB 2 should be extracted and purified.


Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (2/2)

Title: Gel extraction of ~2000bp fragment from today's gel

Start Time: 3:50 PM

Purpose: To extract the PCR product of the norCB gene + iGEM prefix & suffix

Protocol: IBI Gel extraction Exceptions: 1. Milli-Q used instead of Elution Buffer

Products:

Sample Label Description Source Label Quantity
8/3/13 GE norCB norCB w/ iGEM prefix/suffix (~1900 bp) from well 3 Well 3 8/3/13 Gel 1

Results: N/A

Notes:

Stop Time: 4:45 PM

Next: 8/3/13 GE norCB should be ligated into the iGEM vector.


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