IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/02: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Levi Palmer (talk | contribs) (Autocreate 2013/08/02 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue) |
Levi Palmer (talk | contribs) |
||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
== <b>Date: | == <b>Date: 08/02/13</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b> == | ||
<b><span style="font-size:16px;">Title:</span></b> | <b><span style="font-size:16px;">Title:</span></b> PCR of norCB | ||
<b>Start Time:</b> | <b>Start Time:</b> 4:30 PM | ||
<b>Purpose:</b> | <b>Purpose:</b> To PCR norCB and add iGEM prefix&suffix | ||
<b>Protocol:</b> <b>Exceptions:</b> | <b>Protocol:</b> LTM ed. 2 pg 45-47 <b>Exceptions:</b> 1. Final primer concentration <0.1 uM 2. Decreased annealing temperature to 63C 3. Increased elongation time to 5 minutes | ||
<b>Products:</b> | <b>Products:</b> | ||
| Line 21: | Line 21: | ||
! Sample Label !! Description !! Source Label !! Quantity | ! Sample Label !! Description !! Source Label !! Quantity | ||
|- | |- | ||
| | | 8/2/13 A norCB 1 || norCB gene from P. aeruginosa with iGEM prefix and suffix using 7.5 uL template P. aeruginosa || P. aeruginosa 7/30 || 1 | ||
|- | |- | ||
| | | 8/2/13 A norCB 2 || norCB gene from P. aeruginosa with iGEM prefix and suffix using 7.5 uL template P. aeruginosa 2x concentration || P. aerguginosa 7/30 2x in H2O || 1 | ||
|} | |} | ||
<b>Results:</b> | <b>Results:</b> N/A | ||
<b>Notes:</b> | <b>Notes:</b> | ||
<b>Stop Time:</b> | <b>Stop Time:</b> 8:35 PM | ||
<b>Next:</b> | <b>Next:</b> Run samples on a gel. Extract, purify, sequence the better resultant gene product. | ||
Revision as of 13:54, 25 January 2014
 Cyanobacteria Mediated Filtration of Coal Flue Gas
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||
Date: 08/02/13 People in lab: Emily Puleo, Kelsey CrossenTitle: PCR of norCB Start Time: 4:30 PM Purpose: To PCR norCB and add iGEM prefix&suffix Protocol: LTM ed. 2 pg 45-47 Exceptions: 1. Final primer concentration <0.1 uM 2. Decreased annealing temperature to 63C 3. Increased elongation time to 5 minutes Products:
Results: N/A Notes: Stop Time: 8:35 PM Next: Run samples on a gel. Extract, purify, sequence the better resultant gene product.
| |||||||||||||
