IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/02

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(Autocreate 2013/08/02 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
Current revision (16:54, 25 January 2014) (view source)
(Date: MM/DD/YY People in lab: Yourname)
 
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
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== <b>Date: 08/02/13</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b>  ==
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<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
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<b><span style="font-size:16px;">Title:</span></b> PCR of norCB
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<b>Start Time:</b>
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<b>Start Time:</b> 4:30 PM
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<b>Purpose:</b>  
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<b>Purpose:</b> To PCR norCB and add iGEM prefix&suffix
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<b>Protocol:</b> <b>Exceptions:</b>  
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<b>Protocol:</b> LTM ed. 2 pg 45-47 <b>Exceptions:</b> 1. Final primer concentration <0.1 uM 2. Decreased annealing temperature to 63C 3. Increased elongation time to 5 minutes
<b>Products:</b>  
<b>Products:</b>  
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! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity
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| Entry || Entry || Entry || #
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| 8/2/13 A norCB 1 || norCB gene from P. aeruginosa with iGEM prefix and suffix using 7.5 uL template P. aeruginosa || P. aeruginosa 7/30 || 1
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| Entry || Entry || Entry || #
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| 8/2/13 A norCB 2 || norCB gene from P. aeruginosa with iGEM prefix and suffix using 7.5 uL template P. aeruginosa 2x concentration || P. aerguginosa 7/30 2x in H2O || 1
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<b>Results:</b>  
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<b>Results:</b> N/A
<b>Notes:</b>  
<b>Notes:</b>  
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<b>Stop Time:</b>  
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<b>Stop Time:</b> 8:35 PM
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<b>Next:</b>
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<b>Next:</b> Run samples on a gel. Extract, purify, sequence the better resultant gene product.

Current revision

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Date: 08/02/13 People in lab: Emily Puleo, Kelsey Crossen

Title: PCR of norCB

Start Time: 4:30 PM

Purpose: To PCR norCB and add iGEM prefix&suffix

Protocol: LTM ed. 2 pg 45-47 Exceptions: 1. Final primer concentration <0.1 uM 2. Decreased annealing temperature to 63C 3. Increased elongation time to 5 minutes

Products:

Sample Label Description Source Label Quantity
8/2/13 A norCB 1 norCB gene from P. aeruginosa with iGEM prefix and suffix using 7.5 uL template P. aeruginosa P. aeruginosa 7/30 1
8/2/13 A norCB 2 norCB gene from P. aeruginosa with iGEM prefix and suffix using 7.5 uL template P. aeruginosa 2x concentration P. aerguginosa 7/30 2x in H2O 1

Results: N/A

Notes:

Stop Time: 8:35 PM

Next: Run samples on a gel. Extract, purify, sequence the better resultant gene product.



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