IGEM:MIT/2007/Protocols: Difference between revisions
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==DNA Work== | ==DNA Work== | ||
*[[../Notebook/2007-6-12#Mini-prep plasmid DNA (EGFP and NNX)|Mini Prep]] | |||
*[[../Notebook/2007-6-12#PCR_amplification_of_EGFP|PCR Amplification]] | *[[../Notebook/2007-6-12#PCR_amplification_of_EGFP|PCR Amplification]] | ||
*[[../Notebook/2007-6-12#PCR_purify(microcentrifuge)|PCR Purification in a Microcentrifuge]] | *[[../Notebook/2007-6-12#PCR_purify(microcentrifuge)|PCR Purification in a Microcentrifuge]] | ||
Revision as of 06:37, 18 June 2007
General Lab Protocols
- [[../Notebook/2007-6-11#General_Lab_Do_s_and_Don’t_s|General Lab Do's and Don'ts]]
- [[../Notebook/2007-6-12#Nanodrop (~20ng/ul) Protocol|Calculating Optical Density; Nanodrop Protocol]]
- [[../Notebook/2007-6-11#made_1_liter_1%_agarose_solution|Making Agarose Solution]]
- [[../Notebook/2007-6-13#Plating_on_Agar|Plating on Agar]]
- [[../Notebook/2007-6-11#innoculate_5ml_LB/Amp_with_bact._colonies_(EGFP_and_NNX)|Innoculating LB with bacteria]]
DNA Work
- [[../Notebook/2007-6-12#Mini-prep plasmid DNA (EGFP and NNX)|Mini Prep]]
- [[../Notebook/2007-6-12#PCR_amplification_of_EGFP|PCR Amplification]]
- [[../Notebook/2007-6-12#PCR_purify(microcentrifuge)|PCR Purification in a Microcentrifuge]]
- [[../Notebook/2007-6-13#Ligation|Ligation]]
- [[../Notebook/2007-6-13#Purifying_the_DNA|Purifying the DNA]]
- [[../Notebook/2007-6-14#Transformation_Protocol|Transformation]]
