IGEM:MIT/2007/Notebook/2007-8-3: Difference between revisions
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(New page: ===Took Out LC's of I14032, B0014, R0051=== *1ml for glycerol stock *rest miniprepped (for sequencing and 3A ligation) **when spun down, B0014 cells slightly reddish tint?) |
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*rest miniprepped (for sequencing and 3A ligation) | *rest miniprepped (for sequencing and 3A ligation) | ||
**when spun down, B0014 cells slightly reddish tint? | **when spun down, B0014 cells slightly reddish tint? | ||
==Transformations== | |||
*Transformed DH5 alpha with the mer operon and the gfp part to send for sequencing | |||
*PCR mer operon in psb3k3 | |||
*I3500 in psb3k3 | |||
Revision as of 08:01, 3 August 2007
Took Out LC's of I14032, B0014, R0051
- 1ml for glycerol stock
- rest miniprepped (for sequencing and 3A ligation)
- when spun down, B0014 cells slightly reddish tint?
Transformations
- Transformed DH5 alpha with the mer operon and the gfp part to send for sequencing
- PCR mer operon in psb3k3
- I3500 in psb3k3
