IGEM:MIT/2007/Notebook/2007-7-4
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Agenda
- mini-prep pSB3k3 from DB3.1 from incubation yesterday
- streak some DB3.1 for future use
- Pick colonies, incubate liquid cultures of DH(alpha)5 - to be taken out of hot room at 4am tonight
Took out DB3.1 liquid cultures
- Yay, all five tubes have growth
- Took from all five cultures for miniprep
- Will streak from cultures 1, 2, 3
Mini-Prep - Nanodrop of purified pSB3k3
1. 8.4 ng/µL 2. 13.1 ng/µL 3. 6.5 ng/µL 4. 11.2 ng/µL 5. 8.1 ng/µL
Streaked DB3.1
- DB3.1 is the strain that is resistant to the ccdB toxin (
- Streaked three plates
- Left in 37C room to incubate overnight
Incubated liquid cultures of DH5a
- Two 10 mL liquid cultures of singly colony DH5a (picked from last night's incubated plates) were put into the 37C room to grow overnight