IGEM:MIT/2007/Notebook/2007-7-31: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
=Agenda= | |||
#Run mercury assay | #Run mercury assay | ||
#Redo live immunostaining with altered protocol | #Redo live immunostaining with altered protocol | ||
| Line 6: | Line 6: | ||
#Redo transformation of BL21 w/ mer.I13500 in 1AT3 | #Redo transformation of BL21 w/ mer.I13500 in 1AT3 | ||
==Mercury Assay== | |||
==CPX Assay== | |||
Varied cell concentrations: (OD) | |||
* .1 | |||
* .3 | |||
* .5 | |||
* 1 | |||
==Results of CPX Assay== | ==Results of CPX Assay== | ||
Revision as of 15:12, 31 July 2007
Agenda
- Run mercury assay
- Redo live immunostaining with altered protocol
- Enter working parts into BioBricks Registry
- Find constitutive promoter and perform 3A assembly with a RBS and CPX
- Redo transformation of BL21 w/ mer.I13500 in 1AT3
Mercury Assay
CPX Assay
Varied cell concentrations: (OD)
- .1
- .3
- .5
- 1
Results of CPX Assay
-
AHL-6 1
-
AHL-6 2
-
AHL-6 3
-
AHL-7 1
-
AHL-7 2
-
AHL-7 3
-
AHL-8 1
-
AHL-8 2
-
AHL-8 3
-
Control 1
-
Control 2
-
Control 3
