IGEM:MIT/2007/Notebook/2007-6-29: Difference between revisions
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(New page: ===Agenda for Friday, June 29=== *Morning **pBAD pickup **Is OmpA crucial to bacterial cell survival? **Finalize oligos (restriction sites) **Research "red team" stuff -- ion exchange **Br...) |
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Revision as of 22:30, 28 June 2007
Agenda for Friday, June 29
- Morning
- pBAD pickup
- Is OmpA crucial to bacterial cell survival?
- Finalize oligos (restriction sites)
- Research "red team" stuff -- ion exchange
- Brian -- stainless steel, nylon binding?
- Afternoon
- Look for metal inducible promoters (Mer) to fuse to GFP/something FP/whatever
- Order above oligo
- Find/finalize vector (pBAD33?)
- Have everything ordered by the end of today!!
- Question to think about: metal ion diffusion into the cell or binding at the surface?
