IGEM:MIT/2007/Ideas3

Inbox

• Put Links and Papers you find for another category here
• Put your initials next to a link if you're in the process of analyzing it

Mussel Protein

Expressing proteins on E coli cell membranes

Mussel Protein

Mgfp-5 Amino Acid Sequence

 1 sseeykggyy pgntyhyhsg gsyhgsgyhg gykgkyygka kkyyykykns gkykylkkar
61 kyhrkgykky ygggss

/lue

OmpX

1. Mecsas J, Welch R, Erickson JW, and Gross CA. Identification and characterization of an outer membrane protein, OmpX, in Escherichia coli that is homologous to a family of outer membrane proteins including Ail of Yersinia enterocolitica. J Bacteriol. 1995 Feb;177(3):799-804. DOI:10.1128/jb.177.3.799-804.1995 |

[OmpX sequence]

Sequence for CPX

• Total length is 558 bp + length of passenger peptide

1. Native SS - 69 bp

    MKKIACLSALAAVLAFTAGTSVA 

2. Embedded SfiI restriction site - 15 bp

    GQSGQ 

3. Passenger Peptide

    XXXXXXXXXXXXXX 

4. Sequence - 18 bp

    GGQSGQ  

5. S54-F148 - 285 bp

    SGDYNKNQYYGITAGPAYRINDWASIYGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDVGTWIAGVGYRF 

6. Join native C and N - 12 bp

    GGSG 

7. A1-S53 - 159 bp

    ATSTVTGGYAQSDAQGQMNKMGGFNLKYRYEEDNSPLGVIGSFTYTEKSRTAS 

Papers!

2. Samuelson P, Wernérus H, Svedberg M, and Ståhl S. Staphylococcal surface display of metal-binding polyhistidyl peptides. Appl Environ Microbiol. 2000 Mar;66(3):1243-8. DOI:10.1128/AEM.66.3.1243-1248.2000 |
3. Kotrba P, Dolecková L, de Lorenzo V, and Ruml T. Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptides. Appl Environ Microbiol. 1999 Mar;65(3):1092-8. DOI:10.1128/AEM.65.3.1092-1098.1999 |