IGEM:MIT/2007/Heavy Metals: Difference between revisions
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#Brocklehurst03 pmid=12901859 | #Brocklehurst03 pmid=12901859 | ||
</biblio> | </biblio> | ||
*contains sequence for ZntR/CadR and PzntA/PcadA | |||
** Semmie's article on CadC linked with GFP [http://www.springerlink.com/content/d4f4fk82bnh3j9jv/fulltext.pdf] | |||
==Mercury== | ==Mercury== | ||
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<biblio> | <biblio> | ||
#<Hansen00 pmid=11094290> | #<Hansen00 pmid=11094290> | ||
</biblio> | |||
:*Plasmid with Pmer, MerR, and reporter (lux, lac, or gfp) tested in e.coli | :*Plasmid with Pmer, MerR, and reporter (lux, lac, or gfp) tested in e.coli | ||
==Current Industry Filtration Techniques== | ==Current Industry Filtration Techniques== | ||
Mercury: approved by EPA - Coagulation/Filtration; Granular Activated Carbon; Lime softening; Reverse osmosis. | Mercury: approved by EPA - Coagulation/Filtration; Granular Activated Carbon; Lime softening; Reverse osmosis. | ||
Revision as of 07:50, 6 July 2007
Reviews
- A Good Overview (though slightly back-dated): Quantitative assessment of worldwide contamination of air, water and soil by trace metals [1]
- A summary of Pollutants from the EPA [2]
Cadmium
- MCL: 5 ppb
- MCLG: 5 ppb
- Known Carcinogen
- Cadmium Contamination Sites
- Ishihara T, Kobayashi E, Okubo Y, Suwazono Y, Kido T, Nishijyo M, Nakagawa H, and Nogawa K. Association between cadmium concentration in rice and mortality in the Jinzu River basin, Japan. Toxicology. 2001 May 28;163(1):23-8. DOI:10.1016/s0300-483x(01)00367-5 |
- Plachimada, India: [3]
- statistics on Pb, Cd, Cr levels in well water surrounding a Coca-Cola plant (2006)
- pictures too :)
- Most recent(June06) paper on e.coli bioaccumulation of Cd
- Deng X, Yi XE, and Liu G. Cadmium removal from aqueous solution by gene-modified Escherichia coli JM109. J Hazard Mater. 2007 Jan 10;139(2):340-4. DOI:10.1016/j.jhazmat.2006.06.043 |
- Heavy Metal Limits
- Staessen JA, Lauwerys RR, Ide G, Roels HA, Vyncke G, and Amery A. Renal function and historical environmental cadmium pollution from zinc smelters. Lancet. 1994 Jun 18;343(8912):1523-7. DOI:10.1016/s0140-6736(94)92936-x |
- Satarug S, Baker JR, Urbenjapol S, Haswell-Elkins M, Reilly PE, Williams DJ, and Moore MR. A global perspective on cadmium pollution and toxicity in non-occupationally exposed population. Toxicol Lett. 2003 Jan 31;137(1-2):65-83. DOI:10.1016/s0378-4274(02)00381-8 |
- Promoters
- Brocklehurst KR, Megit SJ, and Morby AP. Characterisation of CadR from Pseudomonas aeruginosa: a Cd(II)-responsive MerR homologue. Biochem Biophys Res Commun. 2003 Aug 22;308(2):234-9. DOI:10.1016/s0006-291x(03)01366-4 |
- contains sequence for ZntR/CadR and PzntA/PcadA
- Semmie's article on CadC linked with GFP [4]
Mercury
- MCL: 2 ppb
- MCLG: 2 ppb
- 20 ppb makes the fish mercury buildup significant
- Polluted sites
- Detection (to assess our system):
- Gold Nanoparticles http://www.cah.ucf.edu/news/2005-Mercurynose.php
- Promoter
- Hansen LH and Sørensen SJ. Versatile biosensor vectors for detection and quantification of mercury. FEMS Microbiol Lett. 2000 Dec 1;193(1):123-7. DOI:10.1111/j.1574-6968.2000.tb09413.x |
- Plasmid with Pmer, MerR, and reporter (lux, lac, or gfp) tested in e.coli
Current Industry Filtration Techniques
Mercury: approved by EPA - Coagulation/Filtration; Granular Activated Carbon; Lime softening; Reverse osmosis.
