IGEM:MIT/2006/Notebook/2006-8-7
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Sequencing
- Received (6 samples) with some interesting results - hopefully we remedied and will have complete constructs of both ATF1 and BSMT on 8/8 to begin smell testing
- Sent new sequencing order (42 samples) --- remember that the ATF1s probably have double RBS parts!! and that although we sequenced to check, we did not use them in our 8/7 ligations.
Ligations/Transformations into Top10 cells
- R0040ES + A/TEP + B0032.J45014.B0015(2)XP
- R0040ES + A/TEP + B0030.J45014.B0015(4)XP
- R0040ES + A/TEP + B0030.J45014.B0015(6)XP
- R0040ES + A/TEP + B0030.BSMT.B0015(13)XP
- R0040ES + A/TEP + B0030.BSMT.B0015(14)XP
- R0040ES + A/TEP + B0030.BSMT.B0015(-)XP
- R0040ES + A/TEP + B0032.BSMT.B0015(A)XP
- R0040ES + A/TEP + B0032.BSMT.B0015(B)XP
- osmYSP + B0032.J45014.B0015(2)XP
- osmYSP + B0030.J45014.B0015(6)XP
- osmYSP + B0030.BSMT.B0015(13)XP
- osmYSP + B0030.BSMT.B0015(14)XP
- osmYSP + B0030.BSMT.B0015(-)XP
- osmYSP + B0032.BSMT.B0015(A)XP
- osmYSP + B0032.BSMT.B0015(B)XP
SAGD
- Tried the pchB, pchA and pchBA PCR again with new pchBrev primer
- will run gel to check results on 8/8