IGEM:MIT/2006/Notebook/2006-8-3: Difference between revisions
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#THI3 with SX | #THI3 with SX | ||
#BKB with SX | #BKB with SX | ||
==IAA Ligations== | |||
#BAT2<sub>EP</sub> w/ A/C & A/T BKB | |||
#THI3<sub>SX</sub> w/ psBIAK3<sub>SX</sub>->A/K plate |
Revision as of 14:57, 2 August 2006
Primer To Do
- Order new pchBA primers
Miniprep/Digest To Do
- test osmY-E0840 with plate reader
- Miniprep BSMT.B0015 LC
- Cut BSMT.B0015 with XP
- Miniprep B0030
- Cut B0030 with ES and SP(bkb)
- Miniprep R0040
- Cut R0040 with ES and SP(bkb)
WG Ligations/Transformations To Do
- Gel extract BSMT.B0015XP
- Gel extract B0030SP backbone
- Ligate BSMT.B0015XP and B0030SP backbone-> transform
- Ligate B0030ES with BSMT.B0015XP in A/CEP
- Ligate B0030ES with BSMT.B0015XP in A/TEP
osmY Ligations/Transformations To Do
- osmY and A/CEP and A/TEP
ATF1 Miniprep/Digest To Do
- Miniprep ATF1.B0015 LC
- Cut ATF1.B0015 with XP
- Gel extract some of ATF1.B0015XP
ATF1 Ligation/Transformation To Do
- Ligate ATF1.B0015XP with B0030ES in A/CEP and A/TESP
IAA Digest
- BAT2 with EP, ligate with A/C and A/TEP
- THI3 with SX
- BKB with SX
IAA Ligations
- BAT2EP w/ A/C & A/T BKB
- THI3SX w/ psBIAK3SX->A/K plate