IGEM:MIT/2006/Notebook/2006-8-21

From OpenWetWare
Revision as of 09:28, 21 August 2006 by Skatebro (talk | contribs) (→‎Transformations)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

Ligations

Ligate osmYES with XP cut:

  1. Q99ABC
  2. Q119ABC
  3. Q199ABC
  4. Q219ABC
  5. QE0840ABC

(after running a gel to make sure inverter Q044000 is around)

Mutagenesis

Did mutagenesis on 30-BAT2-30-THI3 to get rid of site in THI3 and on pchBA-15

Transformations

Transform ligations and mutagenesis products

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:MIT/2006/Notebook/2006-8-21&oldid=63046"