IGEM:MIT/2006/Notebook/2006-7-26
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Miniprep B0015
Did 3 minipreps of the terminator for general use
Digest R0040 and B0030(2)
Digested R0040 with E,S and B0030 with E,X
We want to hook up both the promoter and RBS
Digest B0030(2) and CDS+Terminator
Cut B0030(2) with SP and CDS+Term with XP to hook up the two
- will cut B0030(2) tomorrow b/c ran out of miniprep
Digest ATF1 for hooking up to B0030(2)
ATF1 cut with EX, cut again with P to hook up to to SP cut B0030(2)
Cut B0015 with EX
Will hook up later
Cut backbone with EP
EP backbone for inserting the SPP
LIGATIONS
- promoter R0040 + rbs B0030
- promoter R0040 + rbs B0032
- stationary phase promoter osmY + backbone pSB1A3-1
Transformations
Liquid Cultures
End of the day liquid cultures for future use: ATF1, R0040, B0030(2)