IGEM:MIT/2006/Notebook/2006-7-26

Digest R0040 and B0030(2)

Digested R0040 with E,S and B0030 with E,X
We want to hook up both the promoter and RBS

Miniprep B0015

Did 3 minipreps of the terminator for general use

Digest B0030(2) and CDS+Terminator

Cut B0030(2) with SP and CDS+Term with XP to hook up the two

Digest ATF1 for hooking up to B0030(2)

ATF1 cut with EX, cut again with P to hook up to to SP cut B0030(2)

Cut B0015 with EX

Will hook up later

Cut backbone with EP

EP backbone for inserting the SPP

LIGATIONS

1. promoter R0040 + rbs B0030
2. promoter R0040 + rbs B0032
3. stationary phase promoter osmY + backbone pSB1A3-1

Liquid Cultures

End of the day liquid cultures for future use: ATF1, R0040, B0030(2)