IGEM:MIT/2006/Notebook/2006-7-26
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SPP Digest
Digested the annealed SPP again- last time used wrong spin column to cleanup ->don't need to do this. the annealed SPP is already cut with E and P
Digest R0040 and B0030(2)
Digested R0040 with E,S and B0030 with E,X
We want to hook up both the promoter and RBS
Miniprep B0015
Did 3 minipreps of the terminator for general use
Digest B0030(2) and CDS+Terminator
Cut B0030(2) with SP and CDS+Term with XP to hook up the two
Digest ATF1 for hooking up to B0030(2)
ATF1 cut with EX, cut again with P to hook up to to SP cut B0030(2)
Cut B0015 with EX
Will hook up later
Cut backbone with EP
EP backbone for inserting the SPP
