IGEM:MIT/2006/Introduction: Difference between revisions
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==Build== | ==Build== | ||
=== | ===Construction methods=== | ||
*[[BioBricks]] <font color=red>- Day 1, Week 1</font> | *[[BioBricks]] <font color=red>- Day 1, Week 1</font> | ||
*Standard assembly <font color=blue>- Day 2, Week 1</font> | *Standard assembly <font color=blue>- Day 2, Week 1</font> | ||
Line 22: | Line 22: | ||
*Direct synthesis | *Direct synthesis | ||
=== | ===Construction tools=== | ||
*Obtain accounts <font color=red>- Day 1, Week 1</font> | *Registry | ||
*Making a part (both basic and | *#Obtain accounts <font color=red>- Day 1, Week 1</font> | ||
*BLAST | *#Making a part (both basic and composite) <font color=red>- Day 1, Week 1</font> | ||
*Sequence alignments | *#BLAST | ||
*#Sequence alignments | |||
*NCBI Entrez <font color=red>- Day 1, Week 1</font> | |||
*VectorNTI | |||
*Others? | |||
==Test== | ==Test== | ||
*Plate reader characterization (i.e. Receiver) | *Plate reader characterization (i.e. Receiver) | ||
*Flow cytometry (i.e. Screening plasmid) | *Flow cytometry (i.e. Screening plasmid) | ||
Line 38: | Line 41: | ||
===Lab notebooks & documentation=== | ===Lab notebooks & documentation=== | ||
*[[OpenWetWare:Getting started | Getting started with wiki editing]] <font color=red>- Day 1, Week 1</font> | *[[OpenWetWare:Getting started | Getting started with wiki editing]] <font color=red>- Day 1, Week 1</font> | ||
*Populate the official iGEM wiki | *Populate the [http://parts.mit.edu/wiki/index.php/MIT_2006 official MIT iGEM 2006 wiki page] | ||
*The online lab notebook framework | |||
===Searching and reading the literature=== | ===Searching and reading the literature=== | ||
*[[Searching the literature]] <font color=green>- Day 3, Week 1</font> | *[[Searching the literature]] <font color=green>- Day 3, Week 1</font> | ||
===Lab safety | ===Lab safety=== | ||
*Complete the [[Endy:Safety_Training | '''Endy lab safety training''']]. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab. | |||
*Heather will be giving the lab-specific training <font color=red>- Day 1, Week 1</font> | |||
Heather will be giving the lab-specific training <font color=red>- Day 1, Week 1</font> | |||
==Links== | ==Links== |
Latest revision as of 12:14, 2 June 2006
This page contains links to introductory information for the MIT iGEM team.
Design
System Design considerations
- Feasible system scale (numbers of parts, plasmids, chassis types)
- Design tools - models, network diagrams
- Moving from system->devices->parts->DNA specification
- Likelihood of success - protein engineering, cloning, new biological techniques and new science etc.
Abstraction hierarchy & standardization
- Parts - zinc fingers?
- Devices - receiver?, inverter?
- Systems - bacterial photography?
- PoPS and composability
Build
Construction methods
- BioBricks - Day 1, Week 1
- Standard assembly - Day 2, Week 1
- 3A assembly
- In frame assembly
- Direct synthesis
Construction tools
- Registry
- Obtain accounts - Day 1, Week 1
- Making a part (both basic and composite) - Day 1, Week 1
- BLAST
- Sequence alignments
- NCBI Entrez - Day 1, Week 1
- VectorNTI
- Others?
Test
- Plate reader characterization (i.e. Receiver)
- Flow cytometry (i.e. Screening plasmid)
- Microscopy
- RT-PCR and Westerns
Necessary skills
Lab notebooks & documentation
- Getting started with wiki editing - Day 1, Week 1
- Populate the official MIT iGEM 2006 wiki page
- The online lab notebook framework
Searching and reading the literature
- Searching the literature - Day 3, Week 1
Lab safety
- Complete the Endy lab safety training. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab.
- Heather will be giving the lab-specific training - Day 1, Week 1
Links
General introduction to ideas in Synthetic Biology
- Adventures in synthetic biology comic --> Learn about PoPS
- Foundations for engineering biology --> rant by Drew Endy
- MIT iGEM 2004 presentation (.ppt,pdf)
- UT Austin and UCSF 2004 project (coliroids)
- Find descriptions of 2005 projects