IGEM:MIT/2006/Introduction: Difference between revisions
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==Build== | ==Build== | ||
=== | ===Construction methods=== | ||
*[[BioBricks]] <font color=red>- Day 1, Week 1</font> | *[[BioBricks]] <font color=red>- Day 1, Week 1</font> | ||
*Standard assembly <font color=blue>- Day 2, Week 1</font> | *Standard assembly <font color=blue>- Day 2, Week 1</font> | ||
| Line 22: | Line 22: | ||
*Direct synthesis | *Direct synthesis | ||
=== | ===Construction tools=== | ||
*Obtain accounts <font color=red>- Day 1, Week 1</font> | *Registry | ||
*Making a part (both basic and subpart) <font color=red>- Day 1, Week 1</font> | *#Obtain accounts <font color=red>- Day 1, Week 1</font> | ||
*BLAST | *#Making a part (both basic and subpart) <font color=red>- Day 1, Week 1</font> | ||
*Sequence alignments | *#BLAST | ||
*#Sequence alignments | |||
*VectorNTI | |||
*Others? | |||
==Test== | ==Test== | ||
Revision as of 17:28, 25 May 2006
This page contains links to introductory information for the MIT iGEM team.
Design
System Design considerations
- Feasible system scale (numbers of parts, plasmids, chassis types)
- Design tools - models, network diagrams
- Moving from system->devices->parts->DNA specification
- Likelihood of success - protein engineering, cloning, new biological techniques and new science etc.
Abstraction hierarchy & standardization
- Parts - zinc fingers?
- Devices - receiver?, inverter?
- Systems - bacterial photography?
- PoPS and composability
Build
Construction methods
- BioBricks - Day 1, Week 1
- Standard assembly - Day 2, Week 1
- 3A assembly
- In frame assembly
- Direct synthesis
Construction tools
- Registry
- Obtain accounts - Day 1, Week 1
- Making a part (both basic and subpart) - Day 1, Week 1
- BLAST
- Sequence alignments
- VectorNTI
- Others?
Test
Part and device characterization
- Plate reader characterization (i.e. Receiver)
- Flow cytometry (i.e. Screening plasmid)
- Microscopy
- RT-PCR and Westerns
Necessary skills
Lab notebooks & documentation
- Getting started with wiki editing - Day 1, Week 1
- Populate the official iGEM wiki
Searching and reading the literature
- Searching the literature - Day 3, Week 1
Lab safety certification
To complete your online safety training, visit the Endy lab safety training guide. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab.
Heather will be giving the lab-specific training - Day 1, Week 1
Links
General introduction to ideas in Synthetic Biology
- Adventures in synthetic biology comic --> Learn about PoPS
- Foundations for engineering biology --> rant by Drew Endy
- MIT iGEM 2004 presentation (.ppt,pdf)
- UT Austin and UCSF 2004 project (coliroids)
- Find descriptions of 2005 projects
