IGEM:MIT/2005/Techniques

Lab Protocols

• [[../Resuspend Primers To Working Concentration/]]
• [[../Pipetman use, working w/ bacteria, PCR/]]
• [[../Miniprepping DNA, Agarose gels, RE digests/]]
• [[../Excising DNA from gels, purifying DNA from gels/]]
• [[../Ligations, Transformations/]]

Others

• Codon Usage
• Translate Tool
• Orf (open reading frame) Finder
• Amino Acid Codes
• [[../Ray's Tools/]]
• Restriction Sites: http://www.bioscience.org/urllists/restrict.htm
  • EcoR1
    • G AATTC
    • CTTAA G
  • HaeIII
    • GG CC
    • CC GG
  • BamH1
    • G GATCC
    • CCTAG G
  • Pst1
    • CTGCA G
    • G ACGTC
  • EcoKI
    • 5'AAC(N)6GTGC3'
  • EcoBI
    • 5'TCA(N)8TGCT3'
  • Not1
    • 5' GCGGCCGC 3'
  • Xho1
    • 5' C TCGAG 3'

• Base pair finder - read the readme first... if you have any questions, ask me..
  • http://web.mit.edu/ymk/Public/igem/README.txt
  • http://web.mit.edu/ymk/Public/igem/sequen.sh
  • http://web.mit.edu/ymk/Public/igem/ByeNewLine.class
• Epitope Tagging
• QuantumDotfor detection/illuminescence