IGEM:MIT/2005/Techniques: Difference between revisions
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>Jenny No edit summary |
>Ymk (→Others) |
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*[http://www.geneinfinity.org/rastop/manual/aacode.htm Amino Acid Codes] | *[http://www.geneinfinity.org/rastop/manual/aacode.htm Amino Acid Codes] | ||
*[[../Ray's Tools/]] | *[[../Ray's Tools/]] | ||
*Restriction Sites: | *Restriction Sites: http://www.bioscience.org/urllists/restrict.htm | ||
**EcoR1 | **EcoR1 | ||
***G AATTC | ***G AATTC | ||
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**Not1 | **Not1 | ||
***5' GCGGCCGC 3' | ***5' GCGGCCGC 3' | ||
**Xho1 | |||
***5' C TCGAG 3' | |||
*Base pair finder - read the readme first... if you have any questions, ask me.. | *Base pair finder - read the readme first... if you have any questions, ask me.. | ||
Latest revision as of 10:48, 1 August 2005
Lab Protocols
- [[../Resuspend Primers To Working Concentration/]]
- [[../Pipetman use, working w/ bacteria, PCR/]]
- [[../Miniprepping DNA, Agarose gels, RE digests/]]
- [[../Excising DNA from gels, purifying DNA from gels/]]
- [[../Ligations, Transformations/]]
Others
- Codon Usage
- Translate Tool
- Orf (open reading frame) Finder
- Amino Acid Codes
- [[../Ray's Tools/]]
- Restriction Sites: http://www.bioscience.org/urllists/restrict.htm
- EcoR1
- G AATTC
- CTTAA G
- HaeIII
- GG CC
- CC GG
- BamH1
- G GATCC
- CCTAG G
- Pst1
- CTGCA G
- G ACGTC
- EcoKI
- 5'AAC(N)6GTGC3'
- EcoBI
- 5'TCA(N)8TGCT3'
- Not1
- 5' GCGGCCGC 3'
- Xho1
- 5' C TCGAG 3'
- EcoR1
- Base pair finder - read the readme first... if you have any questions, ask me..
- Epitope Tagging
- QuantumDotfor detection/illuminescence
