IGEM:MIT/2005/Techniques: Difference between revisions

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>Aanniev
>Ymk
 
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==Lab Protocols==
==Lab Protocols==
*[[../Resuspend Primers To Working Concentration/]]
*[[../Pipetman use, working w/ bacteria, PCR/]]
*[[../Pipetman use, working w/ bacteria, PCR/]]
*[[../Miniprepping DNA, Agarose gels, RE digests/]]
*[[../Miniprepping DNA, Agarose gels, RE digests/]]
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*[http://www.geneinfinity.org/rastop/manual/aacode.htm Amino Acid Codes]
*[http://www.geneinfinity.org/rastop/manual/aacode.htm Amino Acid Codes]
*[[../Ray's Tools/]]
*[[../Ray's Tools/]]
*Restriction Sites:
*Restriction Sites: http://www.bioscience.org/urllists/restrict.htm
**EcoR1
**EcoR1
***G AATTC
***G AATTC
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**EcoBI
**EcoBI
***5'TCA(N)8TGCT3'  
***5'TCA(N)8TGCT3'  
Retrieved from "http://openwetware.org/index.php?title=IGEM:MIT/2005/Annie%27s_Notes"
**Not1
***5' GCGGCCGC 3'
**Xho1
***5' C TCGAG 3'


*Base pair finder - read the readme first... if you have any questions, ask me..
*Base pair finder - read the readme first... if you have any questions, ask me..

Latest revision as of 10:48, 1 August 2005

Lab Protocols

  • [[../Resuspend Primers To Working Concentration/]]
  • [[../Pipetman use, working w/ bacteria, PCR/]]
  • [[../Miniprepping DNA, Agarose gels, RE digests/]]
  • [[../Excising DNA from gels, purifying DNA from gels/]]
  • [[../Ligations, Transformations/]]

Others

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:MIT/2005/Techniques&oldid=32635"