IGEM:MIT/2005/Techniques: Difference between revisions

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*[[../Excising DNA from gels, purifying DNA from gels/]]
*[[../Excising DNA from gels, purifying DNA from gels/]]
*[[../Ligations, Transformations/]]
*[[../Ligations, Transformations/]]
==Others==
*[http://neelix.molbiol.ox.ac.uk:8080/userweb/cocallag/refdata_html/codonusagetable.shtml Codon Usage]
*[http://us.expasy.org/tools/dna.html Translate Tool]
*[http://www.ncbi.nlm.nih.gov/gorf/gorf.html Orf (open reading frame) Finder]
*[http://www.geneinfinity.org/rastop/manual/aacode.htm Amino Acid Codes]
*[[../Ray's Tools/]]
*Restriction Sites:
**EcoR1
***G AATTC
***CTTAA G
**HaeIII
***GG CC
***CC GG
**BamH1
***G GATCC
***CCTAG G
**Pst1
***CTGCA G
***G ACGTC
*Base pair finder - read the readme first... if you have any questions, ask me..
**http://web.mit.edu/ymk/Public/igem/README.txt
**http://web.mit.edu/ymk/Public/igem/sequen.sh
**http://web.mit.edu/ymk/Public/igem/ByeNewLine.class
*[http://www.roche-applied-science.com/prodinfo_fst.htm?/PROD_INF/MANUALS/epitope/epi_toc.htm Epitope Tagging]
*[http://www.qdots.com/live/index.asp QuantumDot]<font size=1>for detection/illuminescence</font>

Revision as of 15:47, 12 July 2005

Lab Protocols

  • [[../Pipetman use, working w/ bacteria, PCR/]]
  • [[../Miniprepping DNA, Agarose gels, RE digests/]]
  • [[../Excising DNA from gels, purifying DNA from gels/]]
  • [[../Ligations, Transformations/]]

Others

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