IGEM:MIT/2005/Receiver 1: ToxR: Difference between revisions
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Information path ... | Information path ... | ||
# | # Fluoroscein introduced to system (as Fluoroscein+DNAspacer+Fluoroscein), travels into periplasm. | ||
# | # Fluoroscein binds to adjacent ToxR'-scFv fusions. | ||
## Causes ToxR' dimerization | ## Causes ToxR' dimerization | ||
# GFP expressed @ CTX_promoter | # GFP expressed @ CTX_promoter | ||
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People path ... | People path ... | ||
# Maxine gives us | # Maxine gives us Fluoroscein, or identifies something else | ||
# Jenny's scFv binds to Maxine's antigen | # Jenny's scFv binds to Maxine's antigen | ||
# Then, Jenny's scFv induces ToxR' to dimerize | # Then, Jenny's scFv induces ToxR' to dimerize |
Revision as of 19:43, 12 July 2005
Receiver Device #1 -- ToxR
POC
Will
Function
To dimerize in the presence of the input signal and cause gene expression to turn on.
Device Depiction
Information path ...
- Fluoroscein introduced to system (as Fluoroscein+DNAspacer+Fluoroscein), travels into periplasm.
- Fluoroscein binds to adjacent ToxR'-scFv fusions.
- Causes ToxR' dimerization
- GFP expressed @ CTX_promoter
- As a function of ToxR dimerization
People path ...
- Maxine gives us Fluoroscein, or identifies something else
- Jenny's scFv binds to Maxine's antigen
- Then, Jenny's scFv induces ToxR' to dimerize
- The dimerization leads to the expression of Jessica's output @ CTXpromoter or another promoter of her choice.
- Ray makes the whole system look like "we meant to do that."
Device Parts
Current Status
In theory this should work (but we need to get the signal into the periplasm)
- All parts specified
- But, no parts are ready for synthesis
Open Issues/Questions
- Can we get the signal in the periplasm?
- Or, can we adapt or extend the ToxR receiver device so that it accept a signal at the outer membrane.
- Need to design the ToxR/scFv fusion?
- The ToxR sensing system must transition from "concept that ought to work" to "detailed enough plan to actually work." This means cleaning the sequences on parts.mit and finding precedent and/or supreme knowledge on how to squeeze out parts with all of the necassary links to interact well.
- We must first know how to build and then build the ToxR system.
- We must design the experiments and then experiment on the ToxR system
Need Help With
- Going from DNA sequence information, to actual physical genetic material that works inside living cells.