IGEM:MIT/2005/Monday 8/15 Meeting Agenda and Minutes: Difference between revisions
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*This week: | *This week: | ||
*#Complete construction of scFv test construct | *#Complete construction of scFv test construct | ||
*#Begin testing: western blotting. Any favorite protocols? | *#Begin testing | ||
*#*expression (cytoplasmic): western blotting. Any favorite protocols? To plan: talk to team mates and lab members. Timescale? | |||
*#*fluorescein binding: need help designing an experiment. should have done by end of week. | |||
'''Promoter.RBS.scFv''' | '''Promoter.RBS.scFv''' |
Revision as of 22:42, 14 August 2005
General Updates
- Kate: Are we coming into your lab today to do more cleaning? When are you around?
- Team: Info on the Endy lab space:
- Jen will show those who weren't around Friday afternoon where our materials are
- Heather needs to do some lab specific safety training with everyone, ASAP. We'll do it after this meeting, if we can.
SubTeam Updates
Format: what is the progress? what are the issues?
Input: Maxine
Receiver Head-Unit: Jen M
- Taking over for Jenny, unless someone else volunteers to.
- This week:
- Complete construction of scFv test construct
- Begin testing
- expression (cytoplasmic): western blotting. Any favorite protocols? To plan: talk to team mates and lab members. Timescale?
- fluorescein binding: need help designing an experiment. should have done by end of week.
Promoter.RBS.scFv [[../../JennyN Lab Notes#Jenny/]] See Detailed Notes Here. PCR start codon to scFv digest Promoter.RBS BB (recipient) digest scFvs (suffix donor) gel purify ligate promoter.RBS.svFv transform overnights for miniprep identify correct clones (digest), streak plates overnights for freezer stock freezer stocks promoter.RBS.scFv
Receiver/Transmitter 1 - ToxR : Wiki-Will
Receiver/Transmitter 2 - FecA : Annie
Signal Processor: Ray
Actuator
No new updates.