IGEM:MIT/2005/Experiments: Difference between revisions
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1. Receive primers <math>\rightarrow</math> Biobrick by PCR: | 1. Receive primers <math>\rightarrow</math> Biobrick by PCR: | ||
J07005 (phoA), J07006 (malE), J07012-14 (scFv 1-3), J07018 (FecA), J07019 (FecA promoter). | J07005 (phoA), J07006 (malE), J07012-14 (scFv 1-3), J07018 (FecA), J07019 (FecA promoter). | ||
2. Can begin assembly of R0040::B0030, for use in parts J07024-28 (see August wk. 1) | 2. ToxR: | ||
::Can begin assembly of R0040::B0030, for use in parts J07024-28 (see August wk. 1) | |||
::Input Experiments : Verify that flourescin oligo's enter cells. | |||
3. FecA: | |||
::PCR FecA, FecA', FecI, FecR, and FecA promoter | |||
::Assemble fecA promoter with GFP --> test | |||
*<b>July wk 4:</b> | *<b>July wk 4:</b> | ||
FecA: | FecA: Fuse scFv with FecA? | ||
ToxR: | ToxR: | ||
Revision as of 07:59, 15 July 2005
Experimental Timeline
- July wk 3:
1. Receive primers [math]\displaystyle{ \rightarrow }[/math] Biobrick by PCR:
J07005 (phoA), J07006 (malE), J07012-14 (scFv 1-3), J07018 (FecA), J07019 (FecA promoter).
2. ToxR:
- Can begin assembly of R0040::B0030, for use in parts J07024-28 (see August wk. 1)
- Input Experiments : Verify that flourescin oligo's enter cells.
3. FecA:
- PCR FecA, FecA', FecI, FecR, and FecA promoter
- Assemble fecA promoter with GFP --> test
- July wk 4:
FecA: Fuse scFv with FecA? ToxR:
- Aug wk 1:
ToxR: -Receive synthesized J07007 (ctx promoter), J07009 (ToxR') -Begin Assembly of: -J07011 (ctx promoter :: gfp reporter) -J07024-26 (promoter::RBS::ToxR'::scFv 1-3) -J07027-28 (Positive and Negative controls, promoter::RBS::ToxR'::PhoA/MalE)
- Aug wk 2:
- Aug wk 3:
- Aug wk 4:
- Sept wk 1
Experiment Details
[[../Input reception Experiments/]]
[[../Receiver 1 experiments: ToxR/]]
[[../Receiver 2 experiments: FecA/]]
