IGEM:MIT/2005/Annie's Notes: Difference between revisions
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[[Image:1pnz.pdb1-500.jpg|thumb|FecA N-mer]] | [[Image:1pnz.pdb1-500.jpg|thumb|FecA N-mer]] | ||
[http://www.rcsb.org/pdb/cgi/explore.cgi?job=summary&pdbId=1PNZ&page=&pid=91931121117910 PDB page] | [http://www.rcsb.org/pdb/cgi/explore.cgi?job=summary&pdbId=1PNZ&page=&pid=91931121117910 PDB page] | ||
*EcoKI: 5' -AAC(N)6GTGC- 3' | |||
*EcoBI: 5' -TCA(N)8TGCT- 3' | |||
Revision as of 13:00, 14 July 2005
[[../Sensor 2/]]
Have it
- -Flourescein (TK)
- -
Internal Fusion brainstorm
- Phage introducing scFv into cells then homologous recombination
Old Notes
- Fusion: 2 ways
- -Circular permutation generation
- + How is it done? -- Work w/ Drew
- + Ways to screen/select?
- -Specific locations
- + Identify a number of places where scFv can be fused -- Work w/ Tom
- + Specify and characterizations and ordering -- Work w/ Jen
- Conformational change
- -
- Experiments - tests we are running -- Work w/ Ray & Jessica
- -A chart
- -Read outs: testing expression, binding, folding
- EcoKI: 5' -AAC(N)6GTGC- 3'
- EcoBI: 5' -TCA(N)8TGCT- 3'
