IGEM:MIT/2005/Actuator

POC

Jessica

Function

The purpose of the actuator is to convert PoPS -> Cool

Description

Takes in PoPS from signal processor and converts into Action. If no signal processor, take in PoPs from Recievers

Goals

• • Collect the known reporters from both FecA and ToxR studies
  • Make sure reporter parts that are part of plasmids get here
  • Biobrick these parts
  • Test parts by using constitutive promoter

Current Status

• Some parts specified
  • GFP under ctx promoter BBa_J07011
  • GFP under FecA promoter BBa_J07020
• Parts done:
  • GFP with RBS (Strong) BBa_E0840

• [[../Actuators under consideration/]]

Experiments

• put GFP under constitutive reporter --> test biobrick for future use

Open Issues/Questions

• What are people planning to do for experiments to test for the overall signalling of the system?
• Actuator compatable with analog/digital signal
• LVA degradation time
• What is cool?
  • TK has all sorts of luciferase
  • DE knows somebody in MN who has all sort of pigment genes cloned
  • motility?

Sources

FecA promoter sequence:

• Genbank entry
• Ecocyc entry