IGEM:MIT/2005/Actuator: Difference between revisions
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==Experiments== | ==Experiments== | ||
*put GFP under constitutive reporter | |||
*pcr out lacZ | |||
==Open Issues/Questions== | ==Open Issues/Questions== | ||
Revision as of 06:53, 19 July 2005
POC
Jessica
Function
The purpose of the actuator is to convert PoPS -> Cool
Description
Takes in PoPS from signal processor and converts into Action. If no signal processor, take in PoPs from Recievers
Goals
- Collect the known reporters from both FecA and ToxR studies
- Make sure reporter parts that are part of plasmids get here
- Biobrick these parts
- Test parts by using constitutive promoter
Current Status
- Some parts specified
- GFP under ctx promoter BBa_J07011
- GFP under FecA promoter BBa_J07020
- GFP with RBS (Strong) BBa_E0840
- No parts made
- [[../Actuators under consideration/]]
Experiments
- put GFP under constitutive reporter
- pcr out lacZ
Open Issues/Questions
- What are people planning to do for experiments to test for the overall signalling of the system?
- Actuator compatable with analog/digital signal
- LVA degradation time
- What is cool?
- TK has all sorts of luciferase
- DE knows somebody in MN who has all sort of pigment genes cloned
- motility?
Need Help
Sources
FecA promoter sequence:
