IGEM:MIT/2005/Actuator: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
>Jxyu
>Jxyu
Line 24: Line 24:


2 sequences for fecA promoter ... which one to use:
2 sequences for fecA promoter ... which one to use:
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=1181901 Genbank entry]
*[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=1181901 Genbank entry]
[http://biocyc.org/ECOLI/new-image?type=OPERON&object=TU00151 Ecocyc entry]
*[http://biocyc.org/ECOLI/new-image?type=OPERON&object=TU00151 Ecocyc entry]

Revision as of 07:28, 13 July 2005

POC

Jessica

Function

The purpose of the actuator is to convert PoPS -> Cool

Goals

  • Collect the known reporters from both FecA and ToxR studies
  • Make sure reporter parts that are part of plasmids get here
  • Biobrick these parts
  • Test parts by using constitutive promoter

Current Status

  • Some parts specified
  • No parts synthesized
  • [[../Current Actuators/]]

Open Issues/Questions

  • What are people planning to do for experiments to test for the overall signalling of the system?
  • Degrading actuator compatable with analog/digital signal

Need Help

Finding fecA promoter sequence

2 sequences for fecA promoter ... which one to use: