Assembly strategy and Lab-work
Please refer to the overall assembly strategy for details.
Three Lab-teams are currently working in parallel to assemble parts which will be needed for down-stream steps, as soon as the synthesized sequences arrive.
LINKS to lab teams.
- Urgent primers have been ordered on Monday 16th.
- 14 other primers will be required. Design is in progress (pic of word table)!
- Experimental design for XylE e.coli testing!
- Functional testing strategies have been developed and have been indicated in the overall assembly strategy diagram. Need to be written up properly!
- XylE and TEV will not be tagged. In order to be able to analyse their activity, we will compare substrate-turnover rates.
- Strategies for testing of expression etc have to be developed and structured.
- List of parts for submission has to be assembled.
- Gant chart!
- How much synthetic AIP will be required to trigger our test system? (Modellers)
- ComE promoter system - recap on mode of function.