IGEM:Imperial/2010/Strategy

Strategy

Assembly strategy and Lab-work

Please refer to the overall assembly strategy for details.

Three Lab-teams are currently working in parallel to assemble parts which will be needed for down-stream steps, as soon as the synthesized sequences arrive.

LINKS to lab teams.

LINKS to shedule history (?)

Wolfs checklist

Additional primers - work in progress

• Urgent primers have been ordered on Monday 16th.

• 14 other primers will be required. Design is in progress (pic of word table)!

• Experimental design for XylE e.coli testing!

• Functional testing strategies have been developed and have been indicated in the overall assembly strategy diagram. Need to be written up properly!

• XylE and TEV will not be tagged. In order to be able to analyse their activity, we will compare substrate-turnover rates.

• Strategies for testing of expression etc have to be developed and structured.

• List of parts for submission has to be assembled.

• Gant chart!

• How much synthetic AIP will be required to trigger our test system? (Modellers)

• ComE promoter system - recap on mode of function.