IGEM:Imperial/2010/Strategy

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(Wolfs checklist)
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[[Image:PrimersPic1.bmp|thumb|right|600px|x400px| '''Additional primers - work in progress''']]
[[Image:PrimersPic1.bmp|thumb|right|600px|x400px| '''Additional primers - work in progress''']]
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* Urgent primers have been ordered on Monday 16th.
 
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* 14 other primers will be required. Design is in progress (pic of word table)!
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* 14 other primers will be required. Design is in progress (pic of word tabl)- have been ordered (OK)
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* Experimental design for XylE e.coli testing!
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* Experimental design for XylE e.coli testing! (OK)
* Functional testing strategies have been developed and have been indicated in the overall assembly strategy diagram. Need to be written up properly!
* Functional testing strategies have been developed and have been indicated in the overall assembly strategy diagram. Need to be written up properly!
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* XylE and TEV will not be tagged. In order to be able to analyse their activity, we will compare substrate-turnover rates.
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* Strategies for testing of expression etc have to be developed and structured. (OK)
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* Strategies for testing of expression etc have to be developed and structured.
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* List of parts for submission has to be assembled.  
* List of parts for submission has to be assembled.  
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* Gant chart!
 
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* How much synthetic AIP will be required to trigger our test system? (Modellers)
 
* ComE promoter system - recap on mode of function.
* ComE promoter system - recap on mode of function.
[[Image:Primers1.1.docx]]
[[Image:Primers1.1.docx]]

Revision as of 06:44, 10 September 2010

Strategy

Please refer to the assembly strategy for details.

Three Lab-teams are currently working in parallel to assemble parts which will be needed for down-stream steps, as soon as the synthesized sequences arrive.

Key updates:

  • XylE characterization: During the next week (week 9) we will start characterization of XylE. Purification of XylE-His tagged from Jeremy's cultures will be a first step in direction of in-vitro assays. Upon assembly of the GFP-XylE fusion protein this week, protein purification will provide us with both XylE and GFP-XylE for in vitro testing. Important kinetic data will hopefully be obtained during the next 7 days.



Assembly strategy

Assembly strategy version 1.1

Wolfs checklist

Additional primers - work in progress
Additional primers - work in progress
  • 14 other primers will be required. Design is in progress (pic of word tabl)- have been ordered (OK)
  • Experimental design for XylE e.coli testing! (OK)
  • Functional testing strategies have been developed and have been indicated in the overall assembly strategy diagram. Need to be written up properly!
  • Strategies for testing of expression etc have to be developed and structured. (OK)
  • List of parts for submission has to be assembled.
  • ComE promoter system - recap on mode of function.

Image:Primers1.1.docx

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