IGEM:Imperial/2010/Strategy

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: =Strategy= ==Assembly strategy and Lab-work== Please refer to the overall assembly strategy for details. Three Lab-teams are currently working in parallel to assemble parts which will ...)
Line 12: Line 12:
==Wolfs checklist==
==Wolfs checklist==
 +
[[Image:PrimersPic1.bmp]]
* Urgent primers have been ordered on Monday 16th.  
* Urgent primers have been ordered on Monday 16th.  
Line 30: Line 31:
* How much synthetic AIP will be required to trigger our test system? (Modellers)
* How much synthetic AIP will be required to trigger our test system? (Modellers)
-
* ComE promoter system - recap on mode of function.  
+
* ComE promoter system - recap on mode of function.
-
 
+
-
*
+

Revision as of 11:27, 17 August 2010

Strategy

Assembly strategy and Lab-work

Please refer to the overall assembly strategy for details.

Three Lab-teams are currently working in parallel to assemble parts which will be needed for down-stream steps, as soon as the synthesized sequences arrive.

LINKS to lab teams.

LINKS to shedule history (?)

Wolfs checklist

Image:PrimersPic1.bmp

  • Urgent primers have been ordered on Monday 16th.
  • 14 other primers will be required. Design is in progress (pic of word table)!
  • Experimental design for XylE e.coli testing!
  • Functional testing strategies have been developed and have been indicated in the overall assembly strategy diagram. Need to be written up properly!
  • XylE and TEV will not be tagged. In order to be able to analyse their activity, we will compare substrate-turnover rates.
  • Strategies for testing of expression etc have to be developed and structured.
  • List of parts for submission has to be assembled.
  • Gant chart!
  • How much synthetic AIP will be required to trigger our test system? (Modellers)
  • ComE promoter system - recap on mode of function.
Personal tools