IGEM:Imperial/2010/Parts

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Chassis Options

Ideally, we would use B. subtilis because it is a well characterised gram positive bacterium that does quorum sensing using small peptides as autoinducers. These small peptides could be produced as a result of cleavage of a membrane-anchored protein by a protease from the parasite which we want to detect.

However, there are issues regarding the extracellular proteases produced by gram positive bacteria. These could interfere with the system and could result in false positives.

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