IGEM:Imperial/2010/Output module

Effector 1 (Protease)	Effector 2 (Dye,Enz)	Pigment biosynthetic pathways
transcr sigma 54	2 colourless	Billins
Activation (phosphorylation)	Enz-in-pathway short pathways ensure - colourless -> colour	Anthocyanins short pathways ensure - colourless -> colour
Target proteases to use: Piotr, Nick, Kyasha not many AA non-toxic can work in E.Coli quantize speed, efficiency	Protein scaffold: Maddie	Fret pairs as back up for effectors
2C DNA binding prot release

This review article has some useful information on FRET.

James- some suggested papers:

1. Kerppola TK. Complementary methods for studies of protein interactions in living cells. Nat Methods. 2006 Dec;3(12):969-71. DOI:10.1038/nmeth1206-969 |
2. Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods. 2006 Dec;3(12):985-93. DOI:10.1038/nmeth967 |