IGEM:Imperial/2010/Output module: Difference between revisions
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(Moving effectors from fast response page to output page) |
m (removing outdated task assignments) |
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| Line 15: | Line 15: | ||
* ensure - colourless -> colour | * ensure - colourless -> colour | ||
|- | |- | ||
| [[IGEM:Imperial/2010/Fast_Response_module/Target_Proteases | Target proteases ]] to use | | [[IGEM:Imperial/2010/Fast_Response_module/Target_Proteases | Target proteases ]] to use | ||
* not many AA | * not many AA | ||
* non-toxic | * non-toxic | ||
* can work in E.Coli | * can work in E.Coli | ||
* quantize speed, efficiency | * quantize speed, efficiency | ||
| Protein scaffold | | Protein scaffold | ||
|Fret pairs as back up for effectors | |Fret pairs as back up for effectors | ||
|- | |- | ||
Revision as of 01:57, 20 July 2010
| Effector 1 (Protease) | Effector 2 (Dye,Enz) | Pigment biosynthetic pathways |
|---|---|---|
| transcr sigma 54 | 2 colourless | Billins |
| Activation (phosphorylation) | Enz-in-pathway
|
Anthocyanins
|
Target proteases to use
|
Protein scaffold | Fret pairs as back up for effectors |
| 2C DNA binding prot release |
This review article has some useful information on FRET.
James- some suggested papers:
- Kerppola TK. Complementary methods for studies of protein interactions in living cells. Nat Methods. 2006 Dec;3(12):969-71. DOI:10.1038/nmeth1206-969 |
- Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods. 2006 Dec;3(12):985-93. DOI:10.1038/nmeth967 |
