IGEM:Imperial/2010/Output module: Difference between revisions
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*[http://www.nature.com/nmeth/journal/v3/n10/full/nmeth932.html In vivo and in vitro protein solubility assays using split GFP] | *[http://www.nature.com/nmeth/journal/v3/n10/full/nmeth932.html In vivo and in vitro protein solubility assays using split GFP] | ||
*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4HKMM92-3&_user=217827&_coverDate=01%2F13%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1414280714&_rerunOrigin=scholar.google&_acct=C000011279&_version=1&_urlVersion=0&_userid=217827&md5=180870c2ecb405ea5e989cfb7a4293a6 Monitoring of conformational change in maltose binding protein using split green fluorescent protein] | *[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4HKMM92-3&_user=217827&_coverDate=01%2F13%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1414280714&_rerunOrigin=scholar.google&_acct=C000011279&_version=1&_urlVersion=0&_userid=217827&md5=180870c2ecb405ea5e989cfb7a4293a6 Monitoring of conformational change in maltose binding protein using split green fluorescent protein] | ||
* [http://pubs.acs.org/doi/full/10.1021/ac010717k Protein Splicing-Based Reconstitution of Split Green Fluorescent Protein for Monitoring Protein−Protein Interactions in Bacteria: Improved Sensitivity and Reduced Screening Time] | |||
Revision as of 05:28, 28 July 2010
| Effector 1 (Protease) | Effector 2 (Dye,Enz) | Pigment biosynthetic pathways |
|---|---|---|
| transcr sigma 54 | 2 colourless | Bilins |
| Activation (phosphorylation) | Enz-in-pathway
|
Anthocyanins
|
Target proteases to use
|
Protein scaffold | Fret pairs as back up for effectors |
| 2C DNA binding prot release |
This review article has some useful information on FRET.
James- some suggested papers:
- Kerppola TK. Complementary methods for studies of protein interactions in living cells. Nat Methods. 2006 Dec;3(12):969-71. DOI:10.1038/nmeth1206-969 |
- Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods. 2006 Dec;3(12):985-93. DOI:10.1038/nmeth967 |
GFP as output
- In vivo and in vitro protein solubility assays using split GFP
- Monitoring of conformational change in maltose binding protein using split green fluorescent protein
- Protein Splicing-Based Reconstitution of Split Green Fluorescent Protein for Monitoring Protein−Protein Interactions in Bacteria: Improved Sensitivity and Reduced Screening Time
