IGEM:Imperial/2010/Output module
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==GFP as output== | ==GFP as output== | ||
| - | [http://www.nature.com/nmeth/journal/v3/n10/full/nmeth932.html In vivo and in vitro protein solubility assays using split GFP] | + | *[http://www.nature.com/nmeth/journal/v3/n10/full/nmeth932.html In vivo and in vitro protein solubility assays using split GFP] |
| - | [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4HKMM92-3&_user=217827&_coverDate=01%2F13%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1414280714&_rerunOrigin=scholar.google&_acct=C000011279&_version=1&_urlVersion=0&_userid=217827&md5=180870c2ecb405ea5e989cfb7a4293a6 | + | *[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4HKMM92-3&_user=217827&_coverDate=01%2F13%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1414280714&_rerunOrigin=scholar.google&_acct=C000011279&_version=1&_urlVersion=0&_userid=217827&md5=180870c2ecb405ea5e989cfb7a4293a6 Monitoring of conformational change in maltose binding protein using split green fluorescent protein] |
| - | Monitoring of conformational change in maltose binding protein using split green fluorescent | + | |
Revision as of 08:00, 28 July 2010
| Effector 1 (Protease) | Effector 2 (Dye,Enz) | Pigment biosynthetic pathways |
|---|---|---|
| transcr sigma 54 | 2 colourless | Bilins |
| Activation (phosphorylation) | Enz-in-pathway
| Anthocyanins
|
Target proteases to use
| Protein scaffold | Fret pairs as back up for effectors |
| 2C DNA binding prot release |
This review article has some useful information on FRET.
James- some suggested papers:
- Kerppola TK. . pmid:17117150.
- Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. . pmid:17072307.
