IGEM:Imperial/2010/Output module

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==GFP as output==
==GFP as output==
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[http://www.nature.com/nmeth/journal/v3/n10/suppinfo/nmeth932_S1.html In vivo and in vitro protein solubility assays using split GFP]
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[http://www.nature.com/nmeth/journal/v3/n10/full/nmeth932.html In vivo and in vitro protein solubility assays using split GFP]

Revision as of 06:13, 28 July 2010

Effector 1 (Protease) Effector 2 (Dye,Enz) Pigment biosynthetic pathways
transcr sigma 54 2 colourless Bilins
Activation (phosphorylation) Enz-in-pathway
  • short pathways
  • ensure - colourless -> colour
Anthocyanins
  • short pathways
  • ensure - colourless -> colour
Target proteases to use
  • not many AA
  • non-toxic
  • can work in E.Coli
  • quantize speed, efficiency
Protein scaffold Fret pairs as back up for effectors
2C DNA binding prot release

This review article has some useful information on FRET.


James- some suggested papers:

  1. Kerppola TK. . pmid:17117150. PubMed HubMed [1]
  2. Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. . pmid:17072307. PubMed HubMed [2]
All Medline abstracts: PubMed HubMed

GFP as output

In vivo and in vitro protein solubility assays using split GFP

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