IGEM:Imperial/2010/Output module
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(FRET article) |
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| - | ! Pigment biosynthetic pathways | + | ! Effector 1 (Protease) !! Effector 2 (Dye,Enz) !! Pigment biosynthetic pathways |
|- | |- | ||
| - | | Billins | + | | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless || Billins |
|- | |- | ||
| - | + | | [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation|Activation (phosphorylation)]] | |
| - | | | + | | Enz-in-pathway |
| + | * short pathways | ||
| + | * ensure - colourless -> colour | ||
| + | | Anthocyanins | ||
* short pathways | * short pathways | ||
* ensure - colourless -> colour | * ensure - colourless -> colour | ||
|- | |- | ||
| - | + | | [[IGEM:Imperial/2010/Fast_Response_module/Target_Proteases | Target proteases ]] to use: Piotr, Nick, Kyasha | |
| + | * not many AA | ||
| + | * non-toxic | ||
| + | * can work in E.Coli | ||
| + | * quantize speed, efficiency | ||
| + | | Protein scaffold: Maddie | ||
|Fret pairs as back up for effectors | |Fret pairs as back up for effectors | ||
| - | + | |- | |
| + | | 2C DNA binding prot release || || | ||
|} | |} | ||
This [http://www.springerlink.com/content/q34u8r7r7u76h045/fulltext.pdf review article] has some useful information on FRET. | This [http://www.springerlink.com/content/q34u8r7r7u76h045/fulltext.pdf review article] has some useful information on FRET. | ||
| + | |||
| + | |||
| + | James- some suggested papers: | ||
| + | <biblio> | ||
| + | #1 pmid=17117150 | ||
| + | #2 pmid=17072307 | ||
| + | </biblio> | ||
Revision as of 07:38, 19 July 2010
| Effector 1 (Protease) | Effector 2 (Dye,Enz) | Pigment biosynthetic pathways |
|---|---|---|
| transcr sigma 54 | 2 colourless | Billins |
| Activation (phosphorylation) | Enz-in-pathway
| Anthocyanins
|
Target proteases to use: Piotr, Nick, Kyasha
| Protein scaffold: Maddie | Fret pairs as back up for effectors |
| 2C DNA binding prot release |
This review article has some useful information on FRET.
James- some suggested papers:
- Kerppola TK. . pmid:17117150.
- Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. . pmid:17072307.
