IGEM:Imperial/2010/Output module: Difference between revisions
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(FRET article) |
(Moving effectors from fast response page to output page) |
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{| class="wikitable" style="text-align: center; width: | {| class="wikitable" style="text-align: center; width: 50%; height: 170px;" border="1" | ||
|- | |- | ||
! Pigment biosynthetic pathways | ! Effector 1 (Protease) !! Effector 2 (Dye,Enz) !! Pigment biosynthetic pathways | ||
|- | |- | ||
| Billins | | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless || Billins | ||
|- | |- | ||
| [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation|Activation (phosphorylation)]] | |||
| Enz-in-pathway | |||
* short pathways | |||
* ensure - colourless -> colour | |||
| Anthocyanins | | Anthocyanins | ||
* short pathways | * short pathways | ||
* ensure - colourless -> colour | * ensure - colourless -> colour | ||
|- | |- | ||
| [[IGEM:Imperial/2010/Fast_Response_module/Target_Proteases | Target proteases ]] to use: Piotr, Nick, Kyasha | |||
* not many AA | |||
* non-toxic | |||
* can work in E.Coli | |||
* quantize speed, efficiency | |||
| Protein scaffold: Maddie | |||
|Fret pairs as back up for effectors | |Fret pairs as back up for effectors | ||
|- | |||
| 2C DNA binding prot release || || | |||
|} | |} | ||
This [http://www.springerlink.com/content/q34u8r7r7u76h045/fulltext.pdf review article] has some useful information on FRET. | This [http://www.springerlink.com/content/q34u8r7r7u76h045/fulltext.pdf review article] has some useful information on FRET. | ||
James- some suggested papers: | |||
<biblio> | |||
#1 pmid=17117150 | |||
#2 pmid=17072307 | |||
</biblio> | |||
Revision as of 04:38, 19 July 2010
| Effector 1 (Protease) | Effector 2 (Dye,Enz) | Pigment biosynthetic pathways |
|---|---|---|
| transcr sigma 54 | 2 colourless | Billins |
| Activation (phosphorylation) | Enz-in-pathway
|
Anthocyanins
|
Target proteases to use: Piotr, Nick, Kyasha
|
Protein scaffold: Maddie | Fret pairs as back up for effectors |
| 2C DNA binding prot release |
This review article has some useful information on FRET.
James- some suggested papers:
- Kerppola TK. Complementary methods for studies of protein interactions in living cells. Nat Methods. 2006 Dec;3(12):969-71. DOI:10.1038/nmeth1206-969 |
- Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods. 2006 Dec;3(12):985-93. DOI:10.1038/nmeth967 |
