IGEM:Imperial/2010/Output module

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(FRET article)
(Moving effectors from fast response page to output page)
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{| class="wikitable" style="text-align: center; width: 20%; height: 170px;" border="1"
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{| class="wikitable" style="text-align: center; width: 50%; height: 170px;" border="1"
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|+ Table of ideas
 
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   ! Pigment biosynthetic pathways
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   ! Effector 1 (Protease) !! Effector 2  (Dye,Enz) !! Pigment biosynthetic pathways
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   | Billins
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   | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless || Billins
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   | [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation|Activation (phosphorylation)]]
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   | Anthocyanins
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   | Enz-in-pathway
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* short pathways
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* ensure - colourless -> colour
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  | Anthocyanins
* short pathways  
* short pathways  
* ensure - colourless -> colour
* ensure - colourless -> colour
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|-
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  | [[IGEM:Imperial/2010/Fast_Response_module/Target_Proteases | Target proteases ]] to use: Piotr, Nick, Kyasha
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* not many AA
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* non-toxic
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* can work in E.Coli
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* quantize speed, efficiency
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  | Protein scaffold: Maddie
   |Fret pairs as back up for effectors
   |Fret pairs as back up for effectors
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|-
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  | 2C DNA binding prot release || ||
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This [http://www.springerlink.com/content/q34u8r7r7u76h045/fulltext.pdf review article] has some useful information on FRET.
This [http://www.springerlink.com/content/q34u8r7r7u76h045/fulltext.pdf review article] has some useful information on FRET.
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 +
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James- some suggested papers:
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<biblio>
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#1 pmid=17117150
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#2 pmid=17072307
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</biblio>

Revision as of 07:38, 19 July 2010

Effector 1 (Protease) Effector 2 (Dye,Enz) Pigment biosynthetic pathways
transcr sigma 54 2 colourless Billins
Activation (phosphorylation) Enz-in-pathway
  • short pathways
  • ensure - colourless -> colour
Anthocyanins
  • short pathways
  • ensure - colourless -> colour
Target proteases to use: Piotr, Nick, Kyasha
  • not many AA
  • non-toxic
  • can work in E.Coli
  • quantize speed, efficiency
Protein scaffold: Maddie Fret pairs as back up for effectors
2C DNA binding prot release

This review article has some useful information on FRET.


James- some suggested papers:

  1. Kerppola TK. . pmid:17117150. PubMed HubMed [1]
  2. Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. . pmid:17072307. PubMed HubMed [2]
All Medline abstracts: PubMed HubMed
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