IGEM:Imperial/2010/Movie Night

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(Characterization of XylE)
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[[Image:200-600nm spectr.jpg|thumb||300px|center|]]
[[Image:200-600nm spectr.jpg|thumb||300px|center|]]
Spectra of XylE transformed E.coli after addition of catechol assay. The '''broad peak around 380nm wavelength''' arises is due to the presence of the product of the enzymatic reaction involving pyrocatechol and XylE enzyme. This peak if absent if a culture of XylE transformed cells are measured without the addition of catechol
Spectra of XylE transformed E.coli after addition of catechol assay. The '''broad peak around 380nm wavelength''' arises is due to the presence of the product of the enzymatic reaction involving pyrocatechol and XylE enzyme. This peak if absent if a culture of XylE transformed cells are measured without the addition of catechol
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==Kinetics of XylE enzyme==
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[[Image:Michaelis-Menten Curve for XylE.jpg|thumb|450px|left]]
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[[Image:Lineweaver Plot for XylE.jpg|thumb|450px|right]]
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*Km=
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*Vmax=
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==Comparative Kinetics of XylE enzyme and GFP-XylE fusion protein==
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[[Image:XylE Vs Gfp-XylE 1.jpg|thumb|450px|left]][[Image:XylE Vs Gfp-XylE 2.jpg|thumb|450px|right]]

Revision as of 20:50, 14 October 2010

  • A Knight's Tale
  • Kirill: Gone in 60 seconds
  • Piotr: 21
  • Ben: Win a date with Tad Hamilton/The Notebook (or Gattaca)
  • Nicolas: 22 :P

Contents

Characterization of XylE

Identification of optimun wavelength for measuring absorbance

Spectra of XylE transformed E.coli after addition of catechol assay. The broad peak around 380nm wavelength arises is due to the presence of the product of the enzymatic reaction involving pyrocatechol and XylE enzyme. This peak if absent if a culture of XylE transformed cells are measured without the addition of catechol

Kinetics of XylE enzyme

  • Km=
  • Vmax=

Comparative Kinetics of XylE enzyme and GFP-XylE fusion protein

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