IGEM:Imperial/2010/Modelling: Difference between revisions

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==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments1 Experiments for the Output Amplification Model]==
==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments1 Experiments for the Output Amplification Model]==
==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments2 Experiments for the Protein Display Model]==
==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments2 Experiments for the Protein Display Model]==


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<p class="flip">Objectives</p>  
<div id="wrapper">
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<div class="accordionButton"><b>Objectives</b></div>
<p>Here are our daily objectives.</p>
<div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Objectives">Here are our daily objectives.</a><br /><br /></div>
</div>
<div class="accordionButton"><b>Output Amplification Model</b></div>
<p class="flip">Output Amplification Model</p>  
<div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Michaelis_Menten">Model based on Michaelis Menten Kinetics</a><br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Mass_Action">Model based on Law of Mass Action</a><br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Variables1">Variables and Constants</a><br /><br /></div>
<div class="panel">
<div class="accordionButton"><b>Protein Display Model</b></div>
<p>Model based on Michaelis Menten Kinetics</p>
<div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Protein_Display">Protein Display Model</a><br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Variables2">Variables and Constants</a><br /><br /></div>
<p>Model based on Law of Mass Action</p>
<div class="accordionButton"><b>Feedback from Wetlab</b></div>
<p>Variables and Constants for this Model</p>
<div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments1">Experiments for Output Amplification Model</a><br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Modelling">Experiments for Protein Display Model</a><br /></div>
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<p class="flip">Protein Display Model</p>  
<div class="panel">
<p>Protein Display Model</p>
<p>Variables and Constants for this Model</p>
</div>
<p class="flip">Feedback from Wetlab</p>  
<div class="panel">
<p>Experiments for the Output Amplification Model</p>
<p>Experiments for the Protein Display Model</p>
<p></p>
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Revision as of 07:03, 8 September 2010

Objectives

These are our daily objectives.

Output Amplification Model

Model based on Michaelis Menten Kinetics

Comparison between different amplification models (HIV1 and TEV) based on Michaelis Menten kinetics. However, Michaelis Menten kinetics does not apply to our system. Therefore, it had to be modelled from first principle (see below using law of mass action).

A
Model using HIV1
A
Model using TEV

Model based on Law of Mass Action

Comparison between these 3 different models (which are based on the law of mass action): Simple production, 1-step and 2-step amplification

A
Simple production of Dioxygenase
A
1-step amplification
A
2-step amplification

Variables and Constants for this Model

Here are the variables and constants that are used in the Output Amplification Model.

Protein Display Model

Protein Display Model

This is a model showing when the ComD receptor will be activated (after proteins have been cleaved).

Variables and constants for this Model

Here are the variables and constants that are used in the Protein Display Model.

Feedback from Wetlab

Experiments for the Output Amplification Model

Experiments for the Protein Display Model

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background: #FFFF99; display: none; } </style> </head> <body> <div id="wrapper"> <div class="accordionButton"><b>Objectives</b></div> <div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Objectives">Here are our daily objectives.</a><br /><br /></div> <div class="accordionButton"><b>Output Amplification Model</b></div> <div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Michaelis_Menten">Model based on Michaelis Menten Kinetics</a><br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Mass_Action">Model based on Law of Mass Action</a><br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Variables1">Variables and Constants</a><br /><br /></div> <div class="accordionButton"><b>Protein Display Model</b></div> <div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Protein_Display">Protein Display Model</a><br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Variables2">Variables and Constants</a><br /><br /></div> <div class="accordionButton"><b>Feedback from Wetlab</b></div> <div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments1">Experiments for Output Amplification Model</a><br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Modelling">Experiments for Protein Display Model</a><br /></div> </div> </body> </html>

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