IGEM:Imperial/2010/Modelling

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(Experiments for the Protein Display Model)
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=[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Objectives Objectives]=
 
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These are our daily objectives.
 
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=Output Amplification Model=
 
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==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Michaelis_Menten Model based on Michaelis Menten Kinetics]==
 
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Comparison between different amplification models (HIV1 and TEV) based on Michaelis Menten kinetics. However, Michaelis Menten kinetics does not apply to our system. Therefore, it had to be modelled from first principle (see below using law of mass action).
 
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{| class="wikitable" style="text-align: center; width: 100%; height: 170px;"
 
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| [[Image:Slide2.JPG|200px|thumb|center|alt=A|Model using HIV1]]
 
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| [[Image:TEV.jpg|200px|thumb|center|alt=A|Model using TEV]]
 
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|}
 
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==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Mass_Action Model based on Law of Mass Action]==
 
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Comparison between these 3 different models (which are based on the law of mass action): Simple production, 1-step and 2-step amplification
 
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{| class="wikitable" style="text-align: center; width: 100%; height: 170px;"
 
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|[[Image:Simple_production.JPG|200px|thumb|center|alt=A|Simple production of Dioxygenase]]
 
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|[[Image:1-step_amplification.JPG|200px|thumb|center|alt=A|1-step amplification]]
 
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|[[Image:2-step_amplification.JPG|200px|thumb|center|alt=A|2-step amplification]]
 
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|}
 
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==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Variables1 Variables and Constants for this Model]==
 
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Here are the variables and constants that are used in the Output Amplification Model.
 
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=Protein Display Model=
 
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==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Protein_Display Protein Display Model]==
 
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This is a model showing when the ComD receptor will be activated (after proteins have been cleaved).
 
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==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Variables2 Variables and constants for this Model]==
 
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Here are the variables and constants that are used in the Protein Display Model.
 
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=Feedback from Wetlab=
 
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==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments1 Experiments for the Output Amplification Model]==
 
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==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments2 Experiments for the Protein Display Model]==
 
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Revision as of 11:16, 8 September 2010

Objectives
Output Amplification Model

Model based on Michaelis Menten Kinetics
Comparison between different amplification models (HIV1 and TEV) based on Michaelis Menten kinetics. However, Michaelis Menten kinetics does not apply to our system. Therefore, it had to be modelled from first principle (see below using law of mass action).
Model using HIV1 Model using TEV

Model based on Law of Mass Action
Comparison between these 3 different models: Simple production, 1-step and 2-step amplification.
Simple Production 1-step amplification 2-step amplification
Variables and Constants
Here are the variables and constants that are used in the Protein Display Model.

Protein Display Model

Protein Display Model
This is a model showing when the ComD receptor will be activated (after proteins have been cleaved).

Variables and Constants
Here are the variables and constants that are used in the Protein Display Model.

Feedback from Wetlab

Personal tools